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| CHENG,SHU-QUN; HE,JUN-LIN; DONG,YAN-LING; LIU,XUE-QING; DING,YU-BIN; GAO,RU-FEI; TAN,YI; YE,QIAN; TIAN,ZHEN-LING; WANG,YING-XIONG. |
| Calreticulin (CRT), a Ca2+-binding storage protein and chaperone in the endoplasmic reticulum, modulates cell adhesiveness and integrin-dependent Ca2+ signaling. However, the role of CRT during implantation remains poorly understood. In the present study, we characterized the expression of CRT mRNA and the protein in mouse endometria from pregnancy DI to D7. Real-Time PCR and in situ hybridization results showed that the levels of CRT mRNA in the endometria of pregnant mice were significantly higher than those of non-pregnant mice (P<0.05), and increased gradually from pregnancy DI to D4, reaching the máximum level on D4, followed by a plateau from D4 to D7. Using immunofluorescence histochemistry and western blot, changes of CRT expression in the... |
| Tipo: Journal article |
Palavras-chave: Calreticulin; Endometrium; Embryo implantation; Mouse. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400012 |
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| Toledo,Victor; Ramírez,Galia; Valck,Carolina; López,Nandy; Ribeiro,Carolina H; Maldonado,Ismael; Aguilar,Lorena; Lemus,David; Ferreira,Arturo. |
| Angiogenesis is a complex multi-step process of neovascularization arising from preexisting blood vessels whose generation is regulated by pro- and anti-angiogenic factors. Both Trypanosoma cruzi calreticulin (TcCRT) and its human counterpart (HuCRT) are antiangiogenic. This is the first report where the TcCRT and HuCRT anti-angiogenic properties are compared in vivo. In the chick embryonic chorioallantoid membrane assay (CAM) and at equimolar concentrations, TcCRT displayed significantly higher antiangiogenic activities than its human counterpart. LPS had marginal effects at the concentrations present in the recombinant protein preparations and the TcCRT antiangiogenic effects were largely inhibited by specific polyclonal antibodies, thus, reinforcing the... |
| Tipo: Journal article |
Palavras-chave: Calreticulin; T. cruzi; Anti-angiogenesis. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000300004 |
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| AGUILAR,LORENA; RAMÍREZ,GALIA; VALCK,CAROLINA; MOLINA,MARÍA C; ROJAS,ÁLVARO; SCHWAEBLE,WILHELM; FERREIRA,VIVIANA; FERREIRA,ARTURO. |
| Trypanosoma cruzi calreticulin (TcCRT), described in our laboratory, retains several important functional features from its vertebrate homologues. We have shown that recombinant TcCRT inhibits the human complement system when it binds to the collagenous portion of C1q. The generation of classical pathway convertases and membrane attack complexes is thus strongly inhibited. In most T. cruzi-infected individuals, TcCRT is immunogenic and mediates the generation of specific antibodies. By reverting the C1q / TcCRT interaction, a parasite immune evasion strategy, these antibodies contribute to the host / parasite equilibrium. In an in vitro correlate of this situation, we show that the C1q / TcCRT interaction is inhibited by F(ab')2 polyclonal anti-TcCRT IgG... |
| Tipo: Journal article |
Palavras-chave: Calreticulin; C1q; F(ab')2 antibody fragments; Trypanosoma cruzi. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200008 |
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